Waters Hplc System Cleaning Procedure

2 0 scope this procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory.

Waters hplc system cleaning procedure.

When preparing a system to run your method it is always important to prime up the system to ensure you get fresh solvents in all of the required lines. Water flush to clear customer s mobile phases from the system. Responsibility quality control officer executive. Technical assistant 3 2 checking.

Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307. Cleaning decontamination columns general guidelines flow cells general. The water flushes are only needed to remove the previous solvent from the system. Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data.

3 0 responsibility 3 1 doing. Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems. Controlling contamination in lc ms and hplc ms systems 715001307. For information on other acquity uplc cleaning methods refer to the recommended cleaning mixtures for lc table in the con trolling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.

Label bottles correctly with bottle content and filling date expiry date. Use solvent inlet filters to protect the system from incoming particles. Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning. 10 nh4oh in water this removes peg and other.

No injections are needed with that. Water acetonitrile methanol ipa 1 1 1 1 with 2 5 fa. Remove all solvent filters. Place a b c and d seal wash and needle wash lines into a clean.

Use only hplc grade solvents and water filtered through 0 2. Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307. Residues or contaminations may block filters or capillaries. When working with any lc system setting it up and storing it is very important.

To clearly lay down the procedure for cleaning. Reverse osmosis to remove most contaminants b. This sop shall be applicable for the hplc system waters alliances 2690 2695 separation modules. Maintaining your hplc uhplc uplc systems.

To clean the system. Water flush to clean acid from the system. Disconnect the column and attach a union and flow directly to waste. It is necessary to restore the system flow path to a near new level of cleanliness note.